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	Provedor de dados Biol. Res. (2) Autor ALMARZA,CAROLINA (2) BULL,PAULINA (1) CARVAJAL,CRISTIAN (1) CHÁVEZ,RENATO (1) EYZAGUIRRE,JAIME (1) FONCEA,ROCIO (1) LEIGHTON,FEDERICO (1) PEIRANO,ALESSANDRA (1) SCHACHTER,KAREN (1) Mais... Palavra-chave Antioxidants (1) Atherosclerosis (1) Endothelial cell (1) Family 10 endoxylanases (1) Gene expression (1) Gene structure (1) Oxidative stress (1) Penicillium purpurogenum (1) Signal transduction (1) Mais... Tipo do documento Journal article (2) Ano 2001 (1) 2000 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Endothelial cell oxidative stress and signal transduction Biol. Res. FONCEA,ROCIO; CARVAJAL,CRISTIAN; ALMARZA,CAROLINA; LEIGHTON,FEDERICO. Endothelial dysfunction (ED) is an early event in atherosclerotic disease, preceding clinical manifestations and complications. Increased reactive oxygen species (ROS) have been implicated as important mechanisms that contribute to ED, and ROS’s may function as intracellular messengers that modulate signaling pathways. Several intracellular signal events stimulated by ROS have been defined, including the identification of two members of the mitogen activated protein kinase family (ERK1/2 and big MAP kinase, BMK1), tyrosine kinases (Src and Syk) and different isoenzymes of PKC as redox-sensitive kinases. ROS regulation of signal transduction components include the modification in the activity of transcriptional factors such as NFkB and others that result in... Tipo: Journal article Palavras-chave: Oxidative stress; Signal transduction; Gene expression; Endothelial cell; Atherosclerosis; Antioxidants. Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200008 Structure analysis of the endoxylanase A gene from Penicillium purpurogenum Biol. Res. CHÁVEZ,RENATO; ALMARZA,CAROLINA; SCHACHTER,KAREN; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME. Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329... Tipo: Journal article Palavras-chave: Family 10 endoxylanases; Gene structure; Penicillium purpurogenum. Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009 Registros recuperados: 2 Primeira ... 1 ... Última

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